Development of an in-house Lepto-LAT kit for screening and detection of Leptospira infection

Leptospirosis is a common zoonotic disease in the tropics and subtropics. Leptospira infected human without prompt detection treatment will face serious consequences such as acute hepatitis-kidneys, pulmonary hemorrhage which can lead to death. Besides MAT gold standard method, Leptosipra antigens developed by DNA recombination technology have been widely studying applying diagnosis of infection animal. Overcoming disadvantages ELISA complicated protocol, requires highly qualified staff specialized equipment, latex agglutination method has studied used detecting pathogens Salmonella, E. coli, world. The advantages this are simple operation, fast cheap. In previous article, we expressed LigB antigen coli cells successfully purified it affinity chromatography with 98% purity. paper, present process for establishment Lepto-LAT kit detect dogs. This had sensitivity specificity 91.75% 91.57%, respectively.